申志發(fā)，加拿大蒙特利爾大學(xué)博士、研究員，NIH博后，新鄉(xiāng)醫(yī)學(xué)院教授，博士生導(dǎo)師。
研究方向：腫瘤轉(zhuǎn)移分子機(jī)制與靶向治療
在該領(lǐng)域，應(yīng)用活細(xì)胞單分子成像、核酸適配體篩選等核心技術(shù)，取得了多項(xiàng)具有國際先進(jìn)水平，甚至有望在個(gè)別領(lǐng)域具有原創(chuàng)性成果。近五年研究成果先后以第一作者或通訊在ANGEW CHEM INT EDIT、Nature Communications、Genes and Development、Molecular Biology of the Cell, Anal. Chem.等知名學(xué)術(shù)期刊上發(fā)表。
近五年論文
1.HuoXu; Yafeng Zhang; Shuxin Zhang; Changhe Ouyang; Feng Li; Suhong Yu; Xiaofang Geng, Zhifa Shen*, Autonomous table-tennis-motion-type DNA machine for the efficient amplification detection of cancer related gene. Sensors & Actuators: B. Chemical.2019, 281,1016-1022. (JCR一區(qū))
2.Lisha Wang, Ying Han, Shuai Xiao, Sha Lv, Cong Wang, Nan Zhang, Zhengyong Wang,Yongqiong Tang, Hongbo Li, Jianxin Lyu, Huo Xu, Zhifa Shen*.Reverse strand-displacement amplification strategy for rapid detection ofp53 gene. Talanta 2018. 187,365-369 . (JCR二區(qū))
3.Zhihua Gao, Chengwei Wu,Sha Lv,Cong Wang,Nan Zhang,Shuai Xiao,Ying Han,Huo Xu, Yan Zhang,Feng Li,Jianxin Lyu,Zhifa Shen*. Nicking-enhanced rolling circle amplification for sensitive fluorescent detection of cancer-related microRNAs. Analytical and Bioanalytical Chemistry 2018. 18, 1277-82(JCR二區(qū))
4.Zhifa Shen, Feng Li, Yifan Jiang, Chang Chen, Huo Xu, Congcong Li, Zhe Yang, Zaisheng Wu. Palindromic molecule beacon-based cascade amplification for colorimetric detection of cancer genes. Anal. Chem. 2018, 90, 3335-3340. DOI: 10.1021/acs.analchem. 7b04895（JCR一區(qū)）
5.Zhengyong Wang; Feng Li; Yan Zhang; HuiZhao; Huo Xu; Zai-Sheng Wu; Jian-Xin Lyu; Zhi-Fa Shen*.
Sensitive detection of cancer gene based on a nicking-mediated RCA of circular DNA nanomachine. Sensors & Actuators: B. Chemical. 2017, 251: 692-698doi:10.1016/j.snb.2017.05.061（JCR一區(qū)）
6.Dengyou Liu; Qimei Luo; Fawen Deng ; Zhen Li; Benxiang li; Zhifa Shen*. Ultrasensitive electrochemical biosensor based on the oligonucleotide self-assembled monolayer-mediated immunosensing interface. Analytica Chimica Acta.2017, 971: 26-32 ,（JCR一區(qū)）
7.Feng Li, Hui Zhao, Zai-Sheng Wu, Zhe Yang, Huo Xu, Chang Xue,, Jian-xin lyu, Zhi-Fa Shen* Single palindromic molecular beacon-based amplification for genetic analysis of cancers. Biosens Bioelectron. 2017; 91:692-698. doi: 10.1016/j.bios.2017.01.035.（JCR一區(qū)）
8.Xu H, Zhang R, Li F, Zhou Y, Peng T, Wang X, Shen Z*Double-hairpin molecular-beacon-based amplification detection for gene diagnosis linked to cancer. Anal Bioanal Chem. 2016 Sep;408(22):6181-8.
9.Li F, Zhou YY, Peng T, Xu H, Zhang RB, Zhao H, Wang ZY, Lyu JX, Wu ZS, Shen ZF*.Highly sensitive detection of cancer-related genes based on complete fluorescence restoration of a molecular beacon with a functional overhang.Analyst. 2016 Jul 21;141(14):4417-23.（JCR二區(qū)）
10.Liu YH, Jin JL, Wang YZ, Tan Y, Zhou YY, Peng T, Li F, Liang WD, Chartrand P, Jiang YY, ShenZF*.Protrusion-localized STAT3 mRNA promotes metastasis of highly metastatic hepatocellular carcinoma cells in vitro.Acta Pharmacol Sin. 2016 Jun;37(6):805-13.（JCR二區(qū)）
11.Zai-sheng Wu+, Zhifa Shen+, Kha Tram,Bruno J. Salena, and Yingfu Li*Topological DNA Assemblies Containing Identical or Fraternal Twins. ChemBioChem.2016 Jun 16;17(12):1142-5. doi: 10.1002/cbic.201600036. (+ These authors contributed equally to this work)（JCR二區(qū)）
12.Zhifa Shen, Zai-Sheng Wu, Dingran Chang, Wenqing Zhang, Christine Lee, Peter Kim, Bruno Salena, & Yingfu Li. A Catalytic DNA Activated by a Specific Strain of Bacterial Pathogen. Angew Chem Int Ed Engl. 2016, 55 (7): 2431-4. (Hot Paper DOI: 10.1002/anie.201510125)（JCR一區(qū)）
13.Zai-Sheng Wu1?, Zhifa Shen1?,Kha Tram, Yingfu Li.Engineering Interlocking DNA Rings with Minimal Physical Interactions. Nature Communications. 2014, 5:4279 (+ These authors contributed equally to this work)（JCR一區(qū)）
14.Zhifa Shen, Anik St-Denis and Pascal Chartrand. Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to yeast bud. Genes and Development, 2010, 24:1914-1926（JCR一區(qū)）
15.Zhifa Shen, Nicolas Paquin1, Amélie Forget and Pascal Chartrand.Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p. Mol.Bio.Cell, 2009. 20, 2265-2275.（JCR二區(qū)）
主持的科研項(xiàng)目
1 .浙江省自然科學(xué)基金面上項(xiàng)目，LY16C070002，HCCLM3細(xì)胞突出中STAT3 mRNA分子內(nèi)定位元件的分析與鑒定，2016/01-2018/12，10萬元，主持。
2. 新鄉(xiāng)醫(yī)學(xué)院人才啟動(dòng)基金，QTJ15005，肝癌細(xì)胞突出中STAT3mRNA定位機(jī)制， 2017/09-2022/08，300萬元，主持。
3. Mechanisms regulating mRNA localization and translational control. Canadian Institutes of Health Research (CIHR), MOP130325 2014/04-2017/03，200萬元（40萬加拿大元）、主持。
4．省部共建重點(diǎn)項(xiàng)目，突出中STAT3 mRNA定位機(jī)制與肝癌轉(zhuǎn)移， WKJ-ZJ-1723 2017-2020，主持，30萬元。
浙江省自然科學(xué)基金面上項(xiàng)目，識別慢性粒細(xì)胞白血病DNA分子探針的篩選與應(yīng)用研究，LY17H200002，2017-2019，項(xiàng)目負(fù)責(zé)人，10萬元
溫州市重大科技專項(xiàng)項(xiàng)目，微環(huán)境下細(xì)胞突出RNA分子動(dòng)態(tài)交流與肝癌轉(zhuǎn)移，2016Y0852， 2017-2020，主持， 100萬元
